NAD+ (Lyophilized)

NAD+ (Lyophilized)

NAD⁺ (β-nicotinamide adenine dinucleotide, oxidized form) is a coenzyme found in every living cell. Structurally it is two nucleotides joined through a pyrophosphate bridge: one carries adenine, the other nicotinamide, and the nicotinamide ring is where electrons are accepted and released. This listing is the dry, lyophilized form, supplied in 500mg and 1000mg amounts for laboratories that weigh and prepare their own solutions.

Product Specifications

• Product: NAD+ (Lyophilized)
• Amount: 500mg / 1000mg
• Form: Lyophilized powder
• Purity: Third-party tested by an ISO 17025 U.S. laboratory. See the batch Certificate of Analysis (COA) on this page.
• Storage: Keep sealed and refrigerated at 2–8°C (36–46°F), protected from light.
• Ships from: United States, 2-day UPS

Chemical Profile

• Compound class: Dinucleotide coenzyme
• Molecular formula: C₂₁H₂₇N₇O₁₄P₂
• Molecular weight: 663.4 g/mol
• CAS number: 53-84-9

The biochemistry researchers study

NAD⁺ has two distinct jobs, and much of the current literature is about the tension between them.

• Redox carrier: NAD⁺ accepts a hydride to become NADH in glycolysis, the TCA cycle and fatty-acid oxidation. NADH then donates those electrons to complex I of the respiratory chain. In this role the molecule is recycled, not used up.
• Consumed substrate: three enzyme families cleave NAD⁺ and release nicotinamide: the sirtuins (NAD⁺-dependent deacylases), the PARPs (which build ADP-ribose chains during DNA repair), and the NADase/cyclase CD38 and its relative CD157. This consumption means the cell must constantly resynthesize the pool.

Cells make NAD⁺ by three routes: de novo from tryptophan, from nicotinic acid through the Preiss-Handler pathway, and through salvage of nicotinamide, where NAMPT is the rate-limiting enzyme. Precursors such as nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) feed into the salvage arm. Separate cytosolic, nuclear and mitochondrial pools add another layer.

Research topics include measuring NAD⁺/NADH ratios in cells and tissues, sirtuin and PARP enzyme kinetics, CD38 activity, and the decline in tissue NAD⁺ levels reported in aged rodents.

Regulatory status

NAD⁺ is an endogenous metabolite. The material sold here is a research reagent, not an FDA-approved drug product, and it is not for human use.

Quality & Documentation

Every batch of NAD+ is made in small runs for Rogue Compounds and tested by an independent U.S. laboratory for identity (mass spectrometry) and purity (HPLC). Each vial is labeled with its batch number and a QR code that opens the testing lab’s own report for that batch. The same batch Certificate of Analysis is posted on this page and in our COA Library, so you can check one against the other.

Research overview

For a longer look at NAD+, covering where it came from, what cell and animal studies have measured, and what the evidence does not show, read our NAD+ research overview. It is written as background reading, and every study it cites is linked to PubMed.

Selected literature

• Verdin E. NAD⁺ in aging, metabolism, and neurodegeneration. Science. 2015;350(6265):1208-1213. PMID 26785480
• Covarrubias AJ, et al. NAD⁺ metabolism and its roles in cellular processes during ageing. Nat Rev Mol Cell Biol. 2021;22(2):119-141. PMID 33353981
• Katsyuba E, et al. NAD⁺ homeostasis in health and disease. Nat Metab. 2020;2(1):9-31. PMID 32694684

Research Use Only

This product is sold strictly as a research chemical for in-vitro laboratory and analytical use by qualified professionals. It is not a drug, dietary supplement, food, or cosmetic, and it is not intended to diagnose, treat, cure, or prevent any disease. Not for human or veterinary use. Rogue Compounds does not provide dosing, preparation, or usage instructions. The literature on this page is listed for scientific background only and does not describe this product or any use of it.